Beyond the optical limit

Optical imaging has a theoretical limit roughly at one micron, with extra "tricks" let's say 5-600 nanometers. Smaller objects cannot be resolved with optical microscopes. Electron microscopes can give a chance, but only "dead" samples can be eligible. The AFM can circumvent this problem, as it is mechanically senses the studied objects, even single molecules. The following image shows self assembled molecule network.

3D images at single touch

The AFM, produces instantaneous real three dimensional images of the studied object simply upon mechanical gentle consecutive touches. The following images were recorded with no chemical staining or posterior image manipulation abut living endothelial cells. Simply mechanics.
The image below is 60 x 60 x 5µm.

I wonder sometimes, why is this ancient technique neglected in modern biology.  The following image shows real three dimensional record of functional myofibrils. Even the transversal structures can be visualized, or better told "sensed". Image size below is 10 x 10 x 2µm.